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Spilki,Fernando R.; Franco,Ana C.; Rijsewijk,Frans A. M.; Weiblen,Rudi; Flores,Eduardo F.; Roehe,Paulo M.. |
This study aimed the in vitro growth characterization of a previously constructed Brazilian bovine herpesvirus 1.2a with a deletion in the glycoprotein E gene (BHV-1.2a gE-). The plaque sizes, penetration and growth kinetics of the Brazilian BHV-1.2a gE- were studied and compared with the parental virus, as well as with a BHV-1.1 gE- recombinant derived from an European BHV-1.1 strain. No statistical differences were observed between the gE- recombinants and the respective parental viruses penetration assays were performed. When single step growth curves were studied, no statistical differences were observed between gE- and parental viruses. However, it was observed that both gE- viruses were excreted from cells in significantly higher titres at 11 hours... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis; Differential vaccine; In vitro growth characteristics; GE- recombinant; BHV-1.2a; Bovine herpesviruses. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200017 |
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